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Zimmer et al., 2020 - Use of a carbonic anhydrase ca17a knockout to investigate mechanisms of ion uptake in zebrafish (Danio rerio)

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Fig. 5 The effect of Ca17a knockdown on chloride (Cl⁻) uptake in 4-dpf zebrafish (Danio rerio) larvae reared in low-Cl⁻ water and the effect of rearing in low-Cl⁻ water on ca17a protein abundance in 4-dpf larvae. There was an effect of ca17a knockdown and rearing water Cl⁻ concentration on Cl⁻ uptake (A) (two-way ANOVA; Cl⁻ concentration: F = 51.9, P < 0.01, morpholino: F = 110.1, P < 0.01, Cl⁻ concentration × morpholino: F = 13.6, P < 0.01, n = 7). There was an effect of rearing water Cl⁻ concentration on Ca17a protein abundance (B) (Welch’s two-sample t test; t = −2.8, P = 0.02, n = 6). Different letters signify significant (P < 0.05) differences between sham and morpholino larvae within a rearing water Cl⁻ concentration and bars with an asterisk indicate significant effects of rearing water Cl⁻ concentration. Data are presented as means ± SE.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.