Figure 1

Figure 1 Allelic consequence of foxc1-targeted CRISPR/Cas9 mutagenesis: (A) a schematic representation of foxc1a^ua1017 and foxc1b^ua1018 mutations, with 7 and 40 base pair deletions (yellow highlighting) upstream of the DNA-binding Forkhead domain; (B) the predicted sequences of the first 40 amino acids translated from wildtype (WT) and mutant proteins with sequence identity (black highlighting), where the foxc1a^ua1017 allele produces a truncated 39-residue protein with loss of sequence homology from amino acid 10 and the foxc1b^ua1018 allele produces a truncated 28-residue protein with loss of sequence homology from amino acid 19; and (C) PCR genotyping from the gDNA template resolving the respective deletions in foxc1a and foxc1b. (FHD, Forkhead domain; UTR, untranslated region; CDS, coding sequence.).