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Fig. 10 Figure 10 Release of IL-6 and TNF-? from human lung (A549) cells post stimulation with IL-1? in vitro. (A) Cell viability was estimated by Alamar blue assay during a 24-hour period. Results are expressed as mean±SD in triplicate analysis. (B) Cells were pre-treated with different concentration of DSV for either 1 day or 3 days before addition of IL-1? and DSV in the desired concentration. Cell supernatant was used in ELISA for the estimation of IL-6 expression 24 hours post-stimulation. Results are expressed as mean±SD in triplicate analysis. (C) Cell supernatant was used for TNF-? expression as above. Results are expressed as mean±SD ## P<0.001 when compared to the normal control (NC) and ** P<0.01 when compared with the IL-1? stimulated control without any test formulation treatments.