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Figure 2
G3BP1 and G3BP2 suppress mTORC1 in a non-redundant manner and form a heterocomplex
(A) Insulin/aa-stimulated siG3BP2 cells. n = 4.
(B) Quantitation of G3BP2 in (A). Shown are data points and mean ± SEM.
(C) Quantitation of RPS6KB1-pT389 in (A). Data are shown as in (B).
(D) Quantitation of RPS6-pS235/236 in (A). Data are shown as in (B).
(E) Serum/aa-starved G3BP1 KO cells. n = 4.
(F) Quantitation of G3BP2 in (E). Shown are data points and mean ± SEM.
(G) Serum/aa-starved siG3BP1 cells. n = 3.
(H) Quantitation of G3BP2 in (G). Shown are data points and mean ± SEM.
(I) Insulin/aa-stimulated G3BP1 KO cells transfected with MYC-FLAG-G3BP1 or MYC-FLAG-G3BP2 (48 h). n = 3.
(J) Quantitation of RPS6KB1-pT389 in (I). Shown are data points and mean ± SEM.
(K) IP against G3BP2 or mock (rabbit IgG). n = 2.
(L) BiFC. Protein+C-terminal mLumin is indicated first; protein+N-terminal mLumin is indicated second. TL, transmitted light. Scale bar, 100 μm. n = 3.
(M) Quantitation of data in (L). Shown are data points and mean ± SEM.
See also
Reprinted from Cell, 184(3), Prentzell, M.T., Rehbein, U., Cadena Sandoval, M., De Meulemeester, A.S., Baumeister, R., Brohée, L., Berdel, B., Bockwoldt, M., Carroll, B., Chowdhury, S.R., von Deimling, A., Demetriades, C., Figlia, G., Genomics England Research Consortium, de Araujo, M.E.G., Heberle, A.M., Heiland, I., Holzwarth, B., Huber, L.A., Jaworski, J., Kedra, M., Kern, K., Kopach, A., Korolchuk, V.I., van 't Land-Kuper, I., Macias, M., Nellist, M., Palm, W., Pusch, S., Ramos Pittol, J.M., Reil, M., Reintjes, A., Reuter, F., Sampson, J.R., Scheldeman, C., Siekierska, A., Stefan, E., Teleman, A.A., Thomas, L.E., Torres-Quesada, O., Trump, S., West, H.D., de Witte, P., Woltering, S., Yordanov, T.E., Zmorzynska, J., Opitz, C.A., Thedieck, K., G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling, 655-674.e27, Copyright (2021) with permission from Elsevier. Full text @ Cell