Fig. 3

Fig. 3 Fig. 3. Caspase-3 (protease) activity of testis cultured in the presence of 0, 10, and 100 nM of zGnih for 24 h. Following a one-day incubation of the testes, the Caspase-3 activity was measured for every single tissue using the tissue lysate by the EnzChek Caspase-3 Kit#2 with Z-DEVD-R110 substrate (Catalog no. E13184). Reactions were carried out at room temperature, and the fluorescence signal was measured at 496/521 nm (excitation/emission) in the Spectra iMax3 Fluorometer. Data were then normalized by milligrams of the testis, and values are expressed as relative fold change of control (mean ± SEM; n = 6). Results were analyzed by ANOVA followed by Tukey's multiple comparison tests. Values with dissimilar superscripts are significantly different (P ≤ 0.05).