Fig. 2

Fig. 2 Fig. 2. A and D show the effects of zGnih on spermatogenesis after 7-days of zebrafish testis tissue culture. (A and C) Proportion of A undifferentiated spermatogonia (A_und), type A differentiated (A_diff) and type B spermatogonia (SpgB) were determined in the section surface area within the spermatogenic cysts in the absence (dotted line) or presence (black bars) of (A) 10 nM zGnih and (C) 100 nM zGnih. Results were analyzed by Student's T-test (n = 5) and presented as fold changes with respect to the control group (basal). Values are mean ± SEM, and sterisks represent significant difference from the basal shown as dotted line. B and D show incorporation of BrdU (5-Bromo-2′-Deoxyuridine) during the last 6 h of 7-days culture. BrdU labeling index of Aund, Adiff, and SpgB cells were determined in the cultured testes in the absence and presence of 10 and 100 nM zGnih. Bars show the mean ± SEM (n = 5). Each treatment was compared with its own (individual) control (contralateral testis). Asterisks over connected line denote statistical significance between the control and treatment group, using Student's T-test.