ZFIN Image: Palominos et al., 2021, Figure 9

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Figure Caption

Figure 9

The nasal vein as the primary route to the olfactory organ during development. (A)Tg(fli1a:EGFP);Tg(gata1a:DsRed);Tg(mpx:GFP) larva at 5 dpf. Erythrocytes (gata1:DsRed+, red, arrows) are observed within the nasal vein after copper exposure. (B) Tracking of blood flow of 10 erythrocytes circulating within the NV, whole-mount preparation in transmitted light (video of 2 min). Each color represents a different erythrocyte. Direction of movement is represented as a yellow arrow. (C) Laser confocal maximum projection of a 15-dpf Tg(fli1a:EGFP); Tg(lyve1b:DsRed) larva, DAPI (gray). (D) Three-dimensional orthogonal view generated from optic sections (boxed area in C), showing the NV (nasal vein, arrow) positive for fli1a:EGFP and lyve1b:DsRed. The NL (nasal lymphatics, arrow) is positive for lyve1b:DsRed and passes along the ventrolateral region of the OO. Total depth: 230 μm, 2-μm spacing. Scale bars: (A,C) = 100 μm, (B) = 50 μm.

Acknowledgments

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