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Fig. 4 (a) Transposon-mediated En test vector cassette. The cassette contains an En test box flanked by two ITRs, the ITR represents Tol2 inverted repeat sequences, and the En test box contains a minimal gata promoter, GFP, and ?-globin polyA signal flanked with two insulators. INS represents a single insulator; (b?f) Expression patterns of Ens and endogenous dlx1a and dlx2a in TK1; (b) GFP expression of line TK1 at 24 and 48 hpf; (c,d) GFP expression of the embryos injected with plasmids carrying En1 and En2 at 24 and 48 hpf, respectively; (e,f) expression of dlx1a and dlx2a at 24 and 48 hpf, respectively. Red arrows indicate similar patterns in telencephalon (TL).