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Fig. 4

ID: ZDB-IMAGE-210205-13
Publication: Shen et al., 2018 - Enhancer Trapping and Annotation in Zebrafish Mediated with Sleeping Beauty, piggyBac and Tol2 Transposons
All Figures: Figures for Shen et al., 2018

Image

Figure Caption

Fig. 4 (a) Transposon-mediated En test vector cassette. The cassette contains an En test box flanked by two ITRs, the ITR represents Tol2 inverted repeat sequences, and the En test box contains a minimal gata promoter, GFP, and β-globin polyA signal flanked with two insulators. INS represents a single insulator; (b–f) Expression patterns of Ens and endogenous dlx1a and dlx2a in TK1; (b) GFP expression of line TK1 at 24 and 48 hpf; (c,d) GFP expression of the embryos injected with plasmids carrying En1 and En2 at 24 and 48 hpf, respectively; (e,f) expression of dlx1a and dlx2a at 24 and 48 hpf, respectively. Red arrows indicate similar patterns in telencephalon (TL).

Figure Data

Constructs: Tg(en.tk1.1-gata2a:EGFP) , Tg(en.tk1.2-gata2a:EGFP) , Tg(en.sk3.10-gata2a:EGFP) , Tg(en.tk4.7-gata2a:EGFP) , Tg(en.tk4.8-gata2a:EGFP) , Tg(en.sk3.9-gata2a:EGFP) , Tg(en.tk4.3-gata2a:EGFP) , Tg(en.tk4.4-gata2a:EGFP) , Tg(en.tk4.6-gata2a:EGFP)

Acknowledgments

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