Figure 5—figure supplement 1.

Figure 5—figure supplement 1.

(A–D") Magnified views of 10 dpf WT (A–A"), vegfab^-/- (B–B"), vegfab^-/-;vegfc^-/- (C–C"), and vegfab^-/-;vegfd^-/- (D–D") larvae carrying the Tg(kdrl:EGFP) transgene that were immunostained for GFP and Claudin-5 (magenta). In 10 WT fish, Claudin-5 immunoreactivity is prominent in mCP epithelial cells and weakly present in the tissues located ventrally to the DLV and PCeV but is barely detectable in the DLV and PCeV (A–A’). No obvious differences were observed in this low level of Claudin-5 expression in these vessels between WT and any of the vegfab^-/-, vegfab^-/-;vegfc^-/-, and vegfab^-/-;vegfd^-/- larvae that formed these vessels (B–D"). Yellow arrows point to the DLV and white arrows to the PCeV. The number of the animals examined was: n = 65 for WT, n = 50 for vegfab^-/-, n = 26 for vegfab^-/-;vegfc^-/-, and, n = 24 for vegfab^-/-;vegfd^-/- fish. Scale bar: 50 μm.