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Figure 6—figure supplement 3. Co-localization analyses in <italic>TgBAC(vegfab:NTR)</italic>, <italic>TgBAC(vegfc:NTR)</italic>, and <italic>TgBAC(vegfd:NTR)</italic> embryos that carried the <italic xmlns:xlink='http://www.w3.org/1999/xlink'>Tg(sox10:EGFP)</italic> transgene.

(A–A") Confocal maximum projection images of the dorsal views of a 48 hpf Tg(ntla:gal4);Tg(UAS:Kaede);Tg(sox10:mRFP) embryo, showing intermingled distribution of Kaede+ and mRFP+ meningeal cells (n = 7 embryos; 180 Tg(ntla:gal4);Tg(UAS:Kaede)+ cells examined; 1 cell co-localized with Tg(sox10:mRFP)+ cell). Yellow arrows point to some of the Kaede+ meningeal cells that were clearly not mRFP+ even on the maximum projection images. (B–B") Confocal maximum projection images of the dorsal views of a 48 hpf Tg(sox10:EGFP);TgBAC(vegfab:NTR) embryo, showing non-overlapping distribution of EGFP+ and NTR-mCherry+ meningeal cells (n = 12 embryos; 80 TgBAC(vegfab:NTR)+ cells examined; 2 cells co-localized with Tg(sox10:EGFP)+ cells). Yellow arrows point to some of the NTR-mCherry+ meningeal cells that were clearly not EGFP+ even on the maximum projection images. (C–C") Confocal maximum projection images of the dorsal views of a 48 hpf Tg(sox10:EGFP);TgBAC(vegfc:NTR) embryo, showing non-overlapping distribution of EGFP+ and NTR-mCherry+ meningeal cells (n = 4 embryos; 66 TgBAC(vegfc:NTR)+ cells examined; 0 cells co-localized with Tg(sox10:EGFP)+ cells). Yellow arrows point to some of the NTR-mCherry+ meningeal cells that were clearly not EGFP+ even on the maximum projection images. (D–D") Confocal maximum projection images of the dorsal views of a 48 hpf Tg(sox10:EGFP);TgBAC(vegfd:NTR) embryo, showing non-overlapping distribution of EGFP+ and NTR-mCherry+ meningeal cells (n = 4 embryos; 66 TgBAC(vegfd:NTR)+ cells examined; 2 cells co-localized with Tg(sox10:EGFP)+ cells). Yellow arrows point to some of the NTR-mCherry+ meningeal cells that were clearly not EGFP+ even on the maximum projection images. Scale bar: 50 μm.

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