Fgfr1a is important for posterior lateral line development. Lateral views of 48 hpf Tg(ClaudinB::lynGFP) (green) embryos. (A,B) In siblings and control-morpholino injected embryos Priml has reached the tip of the tail and deposited 5 neuromasts. (C)fgfr1a-knockdown embryos show a delayed migration of primordia and a reduced number of neuromasts. (A′-C′) Apical constriction, visualized by staining of Zo-1, occurs under all conditions. Quantification of (D) migration distance, (E) primordium length, (F) number and (G) spacing of neuromasts, respectively. Error bars in bar chart represent standard deviation. Scale bars (A,B) 200 μm, (A′-C″) 20 μm. Abbreviations: Co-Mo, Control-Morpholino; L1-6, lateral (pro)neuromast; pLLGg, lateral line ganglion; pLLN, lateral line nerve; ov, otic vesicle; PrimI, primary posterior primordium.
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