Figure 3

Figure 3

Recorded sublethal morphological effects in D. rerio embryos/larvae after 48, 72, and 96 h of exposure to CAT, 4NC, GUA, 5NG, and 4,6DNG. Negative control: normally developed embryo at (a) 48, (b) 72, and (c) 96 hpf. During exposure period alterations were manifested as: (d) yolk sac edema (arrow); (e) pericardial edema (asterisk), undeveloped tail region (arrow); (f) hatched fish with malformed spine (arrow); (g) underdeveloped tail and necrosis of its apical part (dashed arrow), rare pigments; (h) pericardial edema (asterisk), scoliosis (arrow), necrosis of the apical part of the tail (dashed arrow), rare pigments, not hatched; (i) scoliosis (arrows), blood accumulation in the brain region (dashed arrow); (j) pericardial edema (asterisk), yolk sac edema (arrow), scoliosis (dashed arrow); (k, l) pericardial edema (asterisk); (m) underdeveloped embryo: underdeveloped head (arrow), tail not detached (asterisk), delay or anomaly in the absorption of the yolk sac; (n) pericardial edema (asterisk), blood accumulation (arrow), not hatched; (o) pericardial edema (asterisk), blood clotting (arrow), not hatched; (p) blood accumulation at the yolk sac (arrow); (r) hatched fish with malformed spine; (s) pericardial edema (black asterisk), blood accumulation above the yolk sac (arrow), swelling of the yolk sac (white asterisk), yolk sac edema (dashed arrow), mild scoliosis. Developmental abnormalities were recorded using LAS EZ 3.2.0 digitizing software (https://www.leica-microsystems.com/products/microscope-software/p/leica-las-ez/).