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Fig. 4

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ZDB-IMAGE-210131-8
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Figures for Facchinello et al., 2021
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Fig. 4 Effects of Polg mutation on liver, skeletal muscle and mitochondria.

AD Representative Z projections of 3D confocal images of wt and polgsa9574/sa9574 liver at 8 and 16 dpf. The quantification of liver-specific fluorescence from the lfabp10a:DsRed transgene (E) indicates reduced liver size in 16 dpf mutants. FI Reference bright field images (F, G) and light microscopy analysis of muscle birefringence (H, I) in wt and polgsa9574/sa9574 embryos at 3 dpf. The birefringence quantification (see Methods) shows reduced signal in mutants (J). KN Confocal images of the Tg(Hsa.Cox8a:MLS-EGFP)ia301 mitochondrial marker (mitoEGFP) in wt (K, M) and polgsa9574/sa9574 (L, N) at 16 dpf. The relative quantification of integrated density (O) indicates a reduced mitochondrial mass. P The relative quantification of mtDNA copy number, in wt and polg sa9574 mutants, shows a significant reduction of mtDNA in homozygotes. Data are presented as mean ± SEM and were generated from three different experiments, each containing six embryos per genotype and treatment condition (*p < 0.05; **p < 0.01; N = 6, in triplicate). Q, R TEM analysis of mitochondria in 16 dpf skeletal muscle fibres (sm) of wt and polgsa9574/sa9574 detects vesicular cristae with “honeycomb” arrangement inside the mutated mitochondria (indicated by black arrowheads). S Quantification of aberrant morphologies; ***p < 0.001; N = 8 independent TEM images per condition, in triplicate.

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