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Figure 2 Mortality quantification at different time points comparing the different incubation conditions of the zebrafish embryos. (A) Mortality quantified at 2 dpi. (B) Mortality quantified at 6 dpi. (C) Mortality quantified at 12 dpi. Parameters are expressed as percentages from the total number of embryos assayed for each condition, comparing the incubation of the embryos from 2 dpi to 12 dpi at 28 °C, 34 °C and 36 °C. Error bars represent the standard deviation (SD). One-way ANOVA was performed, and differences were considered significant when * p < 0.05 (n28 °C = 150, n34 °C = 150, n36 °C = 120). The experiment has been performed in triplicate.