ZFIN Image: Lin et al., 2020, Fig 4

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Figure Caption

Fig 4 Validation of newly identified germ granule-enriched transcripts by WMISH.

(A) Visualization map of 13 germ granule-enriched transcripts based on DESeq2. (B) Annotations of the validated transcripts. (C) WMISH of the corresponding transcripts at 2- and 8-cell stages. The white arrows indicate aggregated signals on the vegetal side. Animal pole is up and vegetal pole is down. (D) Double fluorescence in situ hybridization showed that four newly identified transcripts were co-localized with nanos transcripts in the germ granule. The white arrowheads indicate co-localized signals. The white arrows indicate the compact germ granule signals. Embryo outlines are demarcated by dashed lines. The WMISH images show representative expression patterns (> 80%, n ≥ 5).

Acknowledgments

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