ZFIN Image: Warchol et al., 2021, Figure 7

Image

Figure Caption

Figure 7

Depletion of macrophages had minimal impact on hair cell regeneration. Tg(mpeg1:Gal4FF/UAS:NTR-mCherry) fish were treated for 24 h in either 10 mM MTZ or 0.1% DMSO. All fish were then rinsed and incubated for 30 min in 100 μM neomycin. Fish were rinsed again and maintained for an additional 2 h or 48 h. Hair cells and macrophages were immunolabeled and hair cell numbers were quantified from neuromast L5 and the two terminal neuromasts. Images from MTZ-treated fish (A) show the absence of macrophages, but otherwise normal levels of neomycin injury and subsequent regeneration. (B) Normal ototoxicity, macrophage recruitment (arrows), and regeneration in DMSO-treated fish. Quantitative data (C,D) show that hair cell injury and regeneration were nearly-identical in normal and macrophage-depleted fish, although the number of regenerated hair cells in the two terminal neuromasts of MTZ-treated was slightly reduced when compared to DMSO-treated controls (*p = 0.0012; n.s.=p > 0.05).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.