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Figure 7

ID: ZDB-IMAGE-210128-16
Publication: Warchol et al., 2021 - Macrophages Respond Rapidly to Ototoxic Injury of Lateral Line Hair Cells but Are Not Required for Hair Cell Regeneration
All Figures: Figures for Warchol et al., 2021

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Figure Caption

Figure 7

Depletion of macrophages had minimal impact on hair cell regeneration. Tg(mpeg1:Gal4FF/UAS:NTR-mCherry) fish were treated for 24 h in either 10 mM MTZ or 0.1% DMSO. All fish were then rinsed and incubated for 30 min in 100 μM neomycin. Fish were rinsed again and maintained for an additional 2 h or 48 h. Hair cells and macrophages were immunolabeled and hair cell numbers were quantified from neuromast L5 and the two terminal neuromasts. Images from MTZ-treated fish (A) show the absence of macrophages, but otherwise normal levels of neomycin injury and subsequent regeneration. (B) Normal ototoxicity, macrophage recruitment (arrows), and regeneration in DMSO-treated fish. Quantitative data (C,D) show that hair cell injury and regeneration were nearly-identical in normal and macrophage-depleted fish, although the number of regenerated hair cells in the two terminal neuromasts of MTZ-treated was slightly reduced when compared to DMSO-treated controls (*p = 0.0012; n.s.=p > 0.05).

Acknowledgments

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