ZFIN Image: Martínez-Gálvez et al., 2021, Figure 6.

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Figure 6.

MENdel predicts which DNA double-strand breaks likely result in single majority deletions and insertions for likely frameshift loss of function alleles. The confusion matrices display the performance of the prediction of (A) frameshift-inducing PreMAs by MENTHU and (B) insertion frameshifts by Lindel across all 5,885 Cas9-mediated edits from Allen et al. (20). (C) MENdel takes 60bp of sequence context centered at a SpCas9-targetable DSB site to predict single majority deletions (PreMAs) using MENTHU and single majority insertions using Lindel. MENdel offers ∼46% more true-positives of frameshift alleles (197) than MENTHU alone (135).

Acknowledgments

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