ZFIN Image: Evans et al., 2021, Figure 3

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Figure 3

Analysis of HPI axis-associated gene expression within brain extracts by qPCR validates behaviourally determined stress reactivity. qPCR analysis of the HPI axis-associated genes, corticotropin-releasing factor (crf), glucocorticoid receptor α (grα) and mineralocorticoid receptor (mr) in association with zebrafish (a) sex and (b) stress reactivity (N = 96). (c) Stress reactivity had a significant effect on the MR:GR ratio, a key indicator of HPA axis dysfunction (N = 32). The expression of SMAD Specific E3 Ubiquitin Protein Ligase (smurf2) was also analysed through qPCR and was significantly associated with both (d) sex and (e) stress reactivity (N = 32). Box plots indicate median values (middle lines), first and third quartiles (box edges) and minimum and maximum values (whiskers). *p-value < 0.05; **p-value < 0.01.

Acknowledgments

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