Image
Figure Caption

Figure 6—figure supplement 3. Representative fluorescence recovery of Wnt3EGFP in <italic>lrp5</italic> knocked-down embryos at 48 hpf after photobleaching.

(A) Expression of Wnt3EGFP before photobleaching. (B) Photobleached region of Wnt3EGFP. (C) Recovery of fluorescence intensity in the bleached region due to diffusion of molecules from the neighboring unbleached regions. (D) Fluorescence recovery curve for Wnt3EGFP with a recovery time (τfast) of ~150 s and a fraction of mobile component (Fm) of ~0.5. The average global diffusion coefficient (Dglobal) measured for Wnt3EGFP after lrp5 knockdown was 2.8 ± 0.8 μm2/s. Images orientation: anterior to the left. Scale bar 30 μm.

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