Figure 4—figure supplement 1.

Figure 4—figure supplement 1.

(A) Equivalent circuit diagram for the stimulation setup. Electrical resistance comes from the electrodes (measured resistance of ~18.5 MΩ), the resistance to ion flow through the larva itself, and the resistance to flow through the external medium (‘shunt’ pathway of current that does not travel through the larva). Shunt and larval resistance are not directly measured. See Methods for discussion of these different parameters. (B) Estimate of current density in the larva. (Left) Maximum-intensity projection of a larva expressing hsp70:myl9-mApple, which labels all the cells. (Upper right) Cross section through the trunk suggests that the dimensions of the larva in the trunk can be approximated as ~80×130 µm. (Lower right) The larval cross-section is simplified as a rectangle for estimation purposes. Three different possible regions through which current could flow are depicted, and the current density resulting from a 1µA current flowing through each of these regions is shown below each region.