Fig. 6

Fig. 6

Loss of PHF6 mimics reduced Notch activity during human T cell development. (A–C) (left) Flow cytometry analysis and (right) absolute cell counts of control versus PHF6 shRNA transduced or DMSO versus 1 μM GSI treated CD34⁺ thymocytes in OP9-DLL1 cocultures showing (A) the development of CD4⁺CD8β⁺ DP thymocytes after 18 days of coculture, (B) the development of CD3⁺TCRαβ⁺ thymocytes after 25 days of coculture and (C) the development of CD3⁺TCRγδ⁺ thymocytes after 25 days of coculture. Data shows the average of 3 independent experiments and errors bars show SEM. *P < 0.05 (paired t-test).