ZFIN Image: Kondrychyn et al., 2020, Fig. 6

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Figure Caption

Fig. 6 Reassembly of actomyosin network at bleb cortex precedes basal bleb retraction.

The spatiotemporal dynamics Lifeact (actin, a, b; representative images of 17 embryos from 4 independent experiments; secondary bleb formation was observed in 27/100 blebs, retraction in 52/100 blebs and persistent blebs in 27/100 blebs) and Myl9b (non-muscle myosin II, c representative image of 21 retracting blebs from 5 embryos from 2 independent experiments) at the cortex of Marcksl1b-induced bleb are depicted in time-lapse images (i and ii in a, i in b, c), kymographs (iii in a, ii in b, c) and as an average intensity over time (iv in a, iii in b, c). Negative correlation between actin (a) or non-muscle myosin II (c) intensity and bleb area is observed in retracting blebs while little change in actin intensity is observed in blebs that do not retract (b). *, secondary bleb. Arrow, increase in actin or non-muscle myosin II assembly. Scale bar, 2 µm.

Acknowledgments

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