IMAGE

Fig 3

ID
ZDB-IMAGE-201121-26
Source
Figures for Suárez et al., 2020
Image
Figure Caption

Fig 3 Wild-type and mutant drFabp2 structural analysis.

A) SDS-PAGE of purified wild-type (lane 2), 17A29A30A (lane 3) and 17A28A29A (lane 4) drFabp2. The molecular weight of the proteins is indicated in kDa in Standard (lane 1). B) Circular dichroism analysis of wild-type, 17A29A30A and 17A28A29A drFabp2 in the far- (185–235 nm) and near- (250–320 nm) UV spectra. C) wild-type, 17A29A30A and 17A28A29R drFabp2-BODIPY FL C16 binding affinity determination. Fluorescence intensity values were recorded at 510 nm (λex) and 530 nm (λem) using 0.5 μM of each protein.

Acknowledgments
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