ZFIN Image: Moss et al., 2020, Fig. 3

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Figure Caption

Fig. 3

Examples of how GFP-Map1Lc3 transgenic zebrafish line can be used to study autophagy in a skeletal context, a Confocal images of the lower jaw and lower jaw joint of a transgenic CMV:EGFP-map1lc3b zebrafish at 3 days post fertilisation (dpf), immunostained for collagen Type II (red) and anti-GFP for lc3 (green). Scale bar = 50 µm and 10 µm, respectively. b Confocal images of epidermal cells in transgenic CMV:EGFP-map1lc3b zebrafish imaged at 5dpf after treated with 100 µl BafilomycinA1 or DMSO for 16 h followed by 45 min live staining in red LysoTracker dye. Inset boxes show zoom of white checked box. Scale bar = 10 µm

Acknowledgments

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