IMAGE

Fig 1

ID

ZDB-IMAGE-201107-3

Genes

bag3

Publication

Diofano et al., 2020 - Genetic compensation prevents myopathy and heart failure in an in vivo model of Bag3 deficiency

All Figures

Figures for Diofano et al., 2020

Image

Figure Caption

Fig 1 Generation of zebrafish <italic>bag3</italic> knockout by CRISPR/Cas9 gene editing.

(A) Structure of the zebrafish bag3 gene and protein. Exon 2 is the target for the CRISPR/Cas9 gene editing in zebrafish bag3. The CRISPR/Cas9-induced mutation (19 bp deletion) in bag3 is shown in bag3 mutant DNA sequencing chromatogram. The 19 nucleotides deletion in bag3^-/- leads to a frame shift, the introduction of a premature stop codon and thereby the premature termination of Bag3 translation, as demonstrated by the alignment of the Bag3^-/- and Bag3^+/+ aminoacid sequences (only partial aminoacid sequence shown) (B-C) Immunoblot analysis of 72 hpf bag3^+/+ embryo protein lysates compared to lysates obtained from bag3^-/- clutchmates with antibody against zebrafish Bag3. The figure shows one representative immunoblot from three independent experiments (N = 3, mean ± SD, P<0.0001 determined using two-tailed t-test). (D) Quantitative real-time PCR of bag3^+/+ and bag3^-/- embryos at 72 hpf shows significant downregulation of bag3 mRNA levels in bag3^-/- embryos (N = 3, mean ± SD, P = 0.0004 determined using two-tailed t-test).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.