ZFIN Image: Costa et al., 2020, Figure EV5

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Figure Caption

Figure EV5 The 3′<styled-content toggle='no' style='fixed-case'>UTR</styled-content> of <italic>rab13</italic> shows low conservation across species whilst retaining <styled-content toggle='no' style='fixed-case'>mRNA</styled-content> localisation potential

Tg(fli1ep:MCP‐GFPnls) tip cell expressing a control Lyn‐mCherry‐24xMS2 construct.

Percentage identity matrix of rab13 3′UTR orthologue sequences.

Multiple sequence alignment between rab13 3′UTR orthologues. Black boxes indicate absolute nucleotide similarity. The human RAB13 3′UTR localisation element is underlined in green.

Scheme depicts stages of zebrafish ISV sprouting. DA: dorsal aorta; DLAV: dorsal longitudinal anastomotic vessel; HM: horizontal myoseptum; NC: notochord; NT: neural tube.

Data information: white arrows indicate direction of ISV sprouting; yellow dashed line outlines ISV cell borders; scale bars = 10 μm; scale bar in inset = 5 μm (A).

Acknowledgments

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