ZFIN Image: Costa et al., 2020, Figure EV2

Image

Figure Caption

Figure EV2 <styled-content toggle='no' style='fixed-case'>CRISPR</styled-content>‐Cas9 editing of the <italic><styled-content toggle='no' style='fixed-case'>RAB</styled-content></italic> 3′<styled-content toggle='no' style='fixed-case'>UTR</styled-content><italic>in vitro and in vivo</italic> does not generate off‐target mutations

Chromatogram confirming the excision of the LE within RAB13 3′UTR in HUVECs.

List of predicted CRISPR‐Cas9 off‐target genes and RNAseq mismatch detection in CRISPR‐Cas9-derived HUVEC clones (n = 1 each genotype).

Chromatogram confirming the CRISPR‐Cas9-mediated excision of 482‐nt within the rab13 3′UTR in zebrafish embryos.

List of predicted CRISPR‐Cas9 off‐target genes and RNAseq mismatch detection in Tg(kdrl:EGFP) rab13^+/+ and rab13^{∆3′UTR/∆3′UTR} embryos (n = 2 each genotype).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO J.