ZFIN Image: Costa et al., 2020, Figure EV1

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Figure Caption

Figure EV1 Clustering of <styled-content toggle='no' style='fixed-case'>RNA</styled-content>seq datasets defines unexpected cell type‐specific diversity to <styled-content toggle='no' style='fixed-case'>mRNA</styled-content> polarisation

Principal component plot depicting the k‐means clustering analysis of mRNAs enriched across cell protrusion types.

Detail of the heat map shown in Fig 1C representing log₂ fold change (FC) levels (protrusions over cell bodies) of mRNAs present in clusters k 2 and k 7. The corresponding HUVEC log₂ FC levels are shown in parallel.

Top: distribution pattern of mRNAs clustered in k 2. Bottom: smFISH co‐detection of k 2 mRNAs and GAPDH in HUVECs.

Top: distribution pattern of mRNAs clustered in k 7. Bottom: smFISH co‐detection of k 7 mRNAs and GAPDH in HUVECs.

Data information: arrows indicate the orientation of RNA localisation; yellow dashed lines outline cell borders; red circles highlight smFISH spots; scale bars = 20 μm (C, D).

Acknowledgments

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