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Figure 1 Photomicrographs of whole-mounted developing zebrafish showing the presence of SA–β–gal staining. Representative images of 2, 3, 4, 5, 6, 7 and 11 dpf zebrafish are shown. The asterisks indicate the presence of intense SA–β–gal staining in the yolk. Thin arrows indicate the presence of intense SA–β–gal staining in the caudal (cloacal) end of the intestine. Thick black arrows indicate the presence of SA–β–gal staining in the brain. Thick empty arrows indicate the presence of SA–β–gal staining in the spinal cord. Arrowheads indicate the presence of SA–β–gal staining in the liver. Circles indicate the presence of SA–β–gal staining in the oesophagus. Scale bar: 200 μm. Images are a composition of different pictures taken under the microscope overlapped together and modified using the same parameters.