Differential expression of cadherins in ocular motor neurons in Isl1:GFP at 48 hpf.
(A, E) Dorsal views of ocular motor neurons (green) and detection of mRNA expression (magenta) of negative control dapB. (B–D, F–H) Expression of type II cadherins cdh7a and cdh11 and atypical cadherin cdh13 (magenta) in ocular motor neurons (green). Dorsal views show cadherin expression in surrounding tissue and masked by GFP-positive area. Outlines indicate locations of nIII and nIV. White and blue arrows indicate high and low expression, respectively. All images represent snapshots of 3D Imaris visualisations of entire z-stacks. Scale bars (A, C–E) = 20 μm. (B–D) Dorsal views of ocular motor neurons and cadherin expression in surrounding tissue and masked by GFP-positive area. (F–H) Transverse views of ocular motor neurons and masked cadherin expression by GFP-positive area.
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