Downregulation of presynaptic cholinergic gene transcript in larval spinal cord. (A, C) Lateral view of 3–4 and 6-day-old larvae processed by in situ hybridization showing a rostro-caudal gradient for chata. Arrowheads point toward reduced transcript levels in rostral spinal cord. (B, D) chrna3 remain expressed in a uniform pattern along the entire spinal cord. The rostro-caudal expression pattern of the transcript is represented by the colored gradient bar on top of each image. Note that there is no change in transcript levels in the brain. Magnification is the same for all images in (A–D). Scale bar: 200 μm.
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