Figure 6

Figure 6

MEQ targets MTA2 to regulate SerRS expression. (A) Dual-luciferase reporter assay for biotin-conjugated MEQ (MEQ-biotin). Values are the mean ± SEM. ns, not significant, ***P < 0.001 using analysis of variance. (B) Western blots show that MEQ-biotin is as active as MEQ in regulating SerRS expression. (C) MEQ-interacting proteins were pulled down from the MDA-MB-231 cell lysate with streptavidin beads and analyzed by SDS-PAGE and silver staining. The black arrow indicates protein bands that may specifically interact with MEQ. (D) Western blotting to confirm that MTA2 was co-purified with MEQ-biotin. (E) Western blotting to show the knockdown efficiency of shRNA against MTA2 (shMTA2) in MDA-MB-231 cells. (F) Western blotting to show the protein levels of MEQ required to induce SerRS expression in MTA2-silenced (shMTA2) or control (shLacZ) MDA-MB-231 cells.