ZFIN Image: Annunziato et al., 2020, Figure 8.

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Figure Caption

Figure 8.

Exocrine pancreas and endocrine pancreatic beta cell development at 96 h post fertilization (hpf) larvae with AFFF exposure. (A) The area of endocrine pancreatic beta cell islets is graphed. (B) Representative images of normal and fragmented islet morphology in 96 hpf larvae. Separate islet fragments are circled. (C) Pie charts depict the percentage of whole and fragmented beta cell islet morphologies observed. (D) The length of the exocrine pancreas normalized to larval body length is graphed. (E) Individual pancreas lengths are plotted for each treatment group. The 10th (stunted) and 1st (severely stunted) percentile marks are set by control pancreas measurements. (F) Representative images depict a normal exocrine pancreas and severely stunted pancreas in a larva exposed to 4.40×10−4% AFFF. Bars represent mean±SEM. Asterisk (*) indicate p<0.05 compared with 0% AFFF, one-way ANOVA, Tukey’s HSD post hoc test or Fisher’s Exact Test for qualitative assessments. n=6 vials per treatment group. Note: AFFF, aqueous film-forming foam; ANOVA, analysis of variance.

Acknowledgments

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