Figure 3—figure supplement 2.

Figure 3—figure supplement 2.

(A, B) Volcano plots of RNA-seq data from (A) kdrl-positive and negative and (B) pdgfrb-positive and negative cells quantified using V4.3. (A, B) Numbers of differentially expressed genes, along with selected known (A) endothelial or (B) mural cell genes are indicated with green dots. (A, B) Genes with significant differences (padj <0.05) are shown as red (log₂ fold change pos/neg > 1) or blue (log₂ fold changepos/neg <-1). (C–E) Left panels, UCSC browser images of (C) slc7a5 (minus strand), (D) slc2a1a (plus strand), and (E) cspg4 (minus strand) loci showing 3' UTR annotations from V4.3, ENS95, and RefSeq. Mapped depth of RNA-seq reads from indicated cell type on the genome, or assigned to each annotation are indicated, as are 3P-seq features. (C–E) Right panels, log₁₀ normalized expression of (C) slc7a5, (D) slc2a1a, and (E) cspg4 in replicate RNA-seq samples (n = 3) quantified using indicated annotations. Values are normalized with median ratio normalization. (C, D) Values display normal distribution (Shapiro-Wilks test) and analysis of variance revealed statistical significance (slc2a1a, p=0.0160; slc7a5, p=0.0002). Adjusted p-values from Dunnett's multiple comparison tests are indicated. (E) Values are not normally distributed, variance determined by Friedman test (p=0.0278). p-values from Dunn's multiple comparison test are indicated. Error bars represent mean and standard deviation.