Figure 3—figure supplement 1.

Figure 3—figure supplement 1.

(A, B) Annotated UCSC Genome Browser screenshots of (A) cenpq and mrpl39 loci and (B) talgn3b and abhd10b loci. RefSeq, Ens95, Ens99 and V4.3 transcript annotations are shown. Ensembl-annotated transcripts overlapping adjacent locus and leading to misassignment of gene names are indicated. (C) Apparent detected expression for abhd10b and tagln3b in pdgfrb^pos RNA-seq reads (n = 3) using indicated annotation. Error bars denote SEM. The abhd10b transcripts are annotated as belonging to the tagln3b gene in Ensembl95 and 99. This leads to a failure to detect abhd10b when using Ensembl annotations, along with spurious inflation of tagln3b expression levels due to the inclusion of reads that should be mapped to abhd10b. (D) Histogram plot of numbers of transcripts per gene for indicated annotation. The plot is limited to genes with 10 or fewer transcripts. (E) UCSC browser image of the erg locus showing transcripts from indicated annotation and mapped reads from GSE32900.