Figure 1—figure supplement 1—source data 2.

Figure 1—figure supplement 1—source data 2. (A) Log10 average expression (n = 3) for kdrlpos-enriched genes as quantified by each indicated annotation. Each separate plot shows genes identified as kdrlpos-enriched only using or Ens95 or RefSeq. Data are not normally distributed, Wilcoxon matched-pairs signed-rank test, p values are indicated. (B) Venn diagram of intersection for genes with a common NCBI ID in Ens95 and RefSeq found significantly enriched in pdgfrbpos cells (log2 fold change [pdgfrbpos/pdgfrbneg]>1,adjp <0.05) using each annotation. (C, D) Volcano plots showing differentially expressed genes from TgBAC(pdgfrb:citrine)s1010-positive and negative cells identified using RNA-seq reads quantified using (C) RefSeq or (D) Ens95 annotations. Genes with significant differences (padj <0.05) are shown as red (log2 fold change >1) or blue (log2 fold change<-1). Grey dots are genes that fall below these statistical cutoffs; n = 3. Green dots indicate selected genes previously identified as expressed in vascular smooth muscle cells or pericytes (see main text). (E) Plots of commonly annotated genes identified as pdgfrbpos-enriched only by Ens95 with indicated values from Ens95 or RefSeq. (F) Correlation of expression levels from indicated annotation for pdgfrbpos-enriched genes identified selectively as such by Ens95 (maroon) or RefSeq (grey) only (left plot) or in both annotations (right plot). Data are not normally distributed, Spearman correlation, r values are indicated. (G) Log10 average expression (n = 3) for pdgfrbpos-enriched genes as quantified by each indicated annotation. Each separate plot shows genes only identified as pdgfrbpos-enriched using Ens95 or RefSeq. Data are not normally distributed, Wilcoxon matched-pairs signed-rank test, p values are indicated.