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Figure 4 - figure supplement 1 - source data 1

ID: ZDB-IMAGE-200916-22
Publication: Ichino et al., 2020 - Building the vertebrate codex using the gene breaking protein trap library
All Figures: Figures for Ichino et al., 2020

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Figure Caption

Figure 4 - figure supplement 1 - source data 1 Ca2+ transients in <italic>ryr1b+/+</italic> myocytes have higher peak amplitude and are more frequent than in <italic>ryr1bmn0348Gt</italic>/<italic>mn0348Gt</italic> myocytes.

(A) Dot plot comparing peak ∆F/F₀ responses (averaged within cell) between ryr1b^+/+ and ryr1b^mn0348Gt^/mn0348Gt animals. (B) Dot plot representing the number of responses per cell (≥0.05 ∆F/F₀) recorded during the 3 min imaging window in ryr1b^+/+ and ryr1b^mn0348Gt^/mn0348Gt animals. Plots show median with interquartile range. n_ryr1b+/+ = 64 cells, n_{ryr1bmn0348Gt}_/mn0348Gt = 48 cells. Data were compiled from four independent experiments containing at least two animals in each group. p-values determined using the Mann-Whitney U test. Effect size (Cohen’s d)=1.445 (A) and 0.931 (B). Source data can be found in Figure 4—figure supplement 1—source data 1.

Figure 4—figure supplement 1—source data 1.Summary data analyzing the parameters of Ca2+ transients in individual tested cells.

wt = ryr1b^+/+, gbt348hom = ryr1b^mn0348Gt^/mn0348Gt, peak = peak ∆F/F₀, and peaknum = number of transients/responses ≥ 0.05 ∆F/F₀.

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