Figure 5—figure supplement 1.

Figure 5—figure supplement 1.

(A, B) Left, plots showing values from bulk RNA-seq comparison of pdgfrb^pos and pdgfrb^neg cells quantified with Ens95 for (A) cartilage and (B) epidermis genes identified as such selectively in V4.2 by scRNA-seq. Red indicates log₂ fold change >1, padj <0.05. (A, B) Venn diagrams showing the intersection of (A) cartilage and (B) epidermis genes identified as pdgfrb^pos-enriched in bulk RNA-seq using indicated annotation. (C) Examples of epidermis genes identified by scRNA-seq using V4.2, but not Ens95. Left, tSNE plots of epidermis cluster from data quantified with Ens95 or V4.2 showing expression of fbln1, mmp11a, and ptx3a. Right, 3' UTR annotation for each gene from Ens95 or V4.2. Location and read density of scRNA-seq reads are indicated.