Timo AIII suppresses the VEGF-activated VEGF/PI3K/Akt/MAPK signaling pathway in HUVECs. a–c HUVECs were treated with VEGF (50 ng/mL) with or without Timo AIII (2 µM) for 24 h. HUVECs incubated with 0.1% DMSO served as a vehicle control. Phosphorylated and total proteins were detected by Western blotting using the specific antibodies. The ratios of phosphorylated and total proteins were calculated. Data are presented as the fold of the control group. The results are presented as the means ± S.E.M. *P < 0.05 and **P < 0.01 versus the control group. #P < 0.05, ##P < 0.01, and ###P < 0.001 versus the VEGF-treated group
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