ZFIN Image: Zhou et al., 2019, Fig. 7

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Fig. 7

Timo AIII inhibits endothelial cell tube formation in HUVECs. The tube formation ability of HUVECs was examined by a µ-Slide coated with Matrigel. a HUVECs were treated with VEGF (50 ng/mL) and various concentrations (0.5, 1, and 2 µM) of Timo AIII for 6 h. HUVECs incubated with 0.1% DMSO served as a vehicle control and with SU5416 (1 µM) as a positive control. HUVECs were imaged under an inverted microscope. Representative images show the tube formation ability of HUVECs under different treatment conditions. b The statistical graph shows the quantitative analysis of HUVEC tube formation. Data are presented as the loop number per field. c HUVECs were treated with VEGF (50 ng/mL) and various concentrations (0.5, 1, and 2 µM) of Timo AIII for 6 h. Cell viability was tested by the MTT assay. The results are presented as the means ± S.E.M. ^#P < 0.05 versus the control group; **P < 0.01 and ***P < 0.001 versus the VEGF-treated group

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