ZFIN Image: Hudson et al., 2020, Figure 9

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Figure Caption

Figure 9

Berbamine analogs enter hair cells. (A) The dose-response curve for the fluorescent analog (BA-17) demonstrates significant protection from 200 μM neomycin. Hair cells were assessed via DASPEI. Data are normalized to controls. One-way ANOVA, F_(4,49) = 50.22, p < 0.0001. N = 10–12, ****p < 0.0001, ***p < 0.001, bars are ± SEM. (B)Tg(α-tubulin: tdTomato) zebrafish were pretreated with BA-17 for 1 h before imaging on a confocal microscope. Fluorescent analog (green) is present in hair cells (red). Scale bar = 10 μm. (C) AB zebrafish were pretreated with BA-17 for 1 h followed by a 3-min exposure to 50 nM LysoTracker before confocal imaging. Fluorescent analog (green) does overlap with lysosomes (red) in some areas (white arrowheads). Scale bars in (B,C) = 10 μm.

Acknowledgments

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