Figure 4

Figure 4

Hair cells are viable after analog exposure. (A) Zebrafish were pretreated with the nuclear marker YO-PRO-1 for 1 h followed by treatment with the optimally protective concentration of each analog for 24 h. Zebrafish were then rinsed in embryo medium (EM) and treated with FM 1–43FX to assess mechanoelectrical transduction (MET) channel function after prolonged analog exposure and removal. Analog treatment did not alter FM 1–43FX or YO-PRO-1-labeling. Scale bar = 10 μm. (B) Examples of Tg(myo6b:EGFP) neuromasts treated with the optimally protective concentration of each analog for 24 h before hair cell assessment. In each case, neuromast morphology appeared normal. Scale bar = 10 μm. (C) Zebrafish were exposed to the optimal protective concentration (OPC) of berbamine analog for 24 h before hair cell assessment with DASPEI. None of the analogs impact hair cell survival. Data are normalized to controls and analyzed by one-way ANOVA, F_(4,48) = 0.6564, p = 0.6252. N = 11–12, bars are ± SEM.