Figure 4—figure supplement 1.

Figure 4—figure supplement 1.

mRNA expression in HEK293T cells upon TBX3 overexpression (black bars) is measured via qRT-PCR and compared to control condition (CTRL, transfection with empty vector, white bars). Consistently with the STF/Luciferase assay (Figure 4C), TBX3 represses the transcription of endogenous Wnt targets. Upon suboptimal Wnt stimulation however (achieved via addition of 1 μM of CHIR), AXIN2 and NKD1 are not (or only mildly) activated in CTRL condition. On the other hand, TBX3 overexpression induces a substantial statistical increase in AXIN2 and NKD1 mRNA abundance when compared to their background level; the Wnt-ON (CHIR 1 μM) condition is compared to the Wnt-OFF basal condition (ON/OFF ration; right panel). The data are presented as the mean ± SD of three independent experiments. Parametric unpaired Student t-test was used to measure the statistical significance (p-values above the charts). GAPDH mRNA was used to normalize transcript levels.