ZFIN Image: Zhou et al., 2020, Figure 4

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Figure Caption

Figure 4

Functional Clustering of Dendritic ROIs

(A–F) Dendritic ROIs from across the entire eye were clustered based on their four spectral kernels (STAR Methods) to yield a total of n = 15 functional clusters that comprised a minimum of 10 ROIs. Shown are heatmaps of red, green, blue, and UV kernels (A, from left to right, respectively) and associated mean chirp response (B), with each entry showing a single ROI, followed by each cluster’s Eye-IPL projection (C), each mean kernel (D), max-scaled kernels superimposed (E), and the mean chirp response (F). Error shadings in SD are shown. For clarity, low-amplitude mean kernels were omitted from column (E). Note that C₁₁^∗ comprised a mixture of responses and may comprise a variety of low-n functional RGC types. Grayscale color maps (A–C) were linearly equalized by hand to maximize subjective discriminability of the full response range across the population of all recordings in a dataset. Lighter grays indicate higher activity/kernel amplitudes. For corresponding data on somata, see Figure S3.

(G) Summary of cluster distributions across the eye, irrespective of IPL depths, for dendritic (top) and somatic (bottom) clusters, scaled by their relative abundance (in %; see scale bars). Eye-distribution profiles were manually allocated to one of the following groups based on which part of visual space is mainly surveyed: SZ (dendritic C₁; somatic C₂); forward (dendritic C₅; somatic C₃); outward (dendritic C_3,9; somatic C_9,11); horizon (dendritic C_2,11; somatic C₁,₄,₁₀); up (dendritic C_4–8; somatic C₇); and down (dendritic C_10,12–15; somatic C₁₂,₁₃). Two large clusters (somatic C_5,8) did not obviously fit to any of these categories and were instead grouped separately as “mixed.” It is possible that these clusters comprise several smaller groups of functional RGCs with distinct eye-wide distributions.

(H) As (E) for both dendritic (top) and somatic (bottom) data, but with all spectral kernels in each waveband superimposed. Note kinetic similarities across most red and green kernels and near complete absence of positive deflections in blue kernels.

Acknowledgments

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