Figure 3

Figure 3

Diverse Color Opponencies in RGCs

(A) Each dendritic (top) and somatic (bottom; inverted y axis) ROI that passed a minimum response criterion (STAR Methods) was allocated to a single bin in a ternary classification scheme according to the relative polarities of their four spectral kernels (3 response states On, Off, and no response) raised to the power of 4 spectral channels (red, green, blue, and UV): 3⁴ = 81 possible combinations. The central row between the bar graphs indicates each bin’s spectral profile: “On” (red, green, blue, and UV); “Off” (black in the respective row); and no response (white in the respective row). For example, the leftmost group, which comprised the highest number of dendritic ROIs, corresponds to ROIs displaying Off kernels in red, green, and blue, with UV showing no response. The bar graphs are color coded as follows: dark gray (non-opponent Off); light gray: (non-opponent On); and orange/brown (opponent). Brown bins indicate opponent bins that are only classified as opponent because they comprise a Blue-Off component (see main text). The horizontal insets summarize all ternary response groups that exceeded a minimum size (indicated by the dashed line) across the following categories: Off; On; and Opponents, here divided into types of spectral computations as indicated by the color circles; two-color symbols denote “simple” opponencies (single spectral zero crossing, e.g., red versus green) between the indicated wavebands (red, green, blue, and UV), although the “flower” symbol denotes complex opponencies (>1 spectral zero crossing, e.g., red and blue versus green).

(B) Maximum-amplitude scaled average kernels of the ten most abundant spectral classes among dendrites in (A).

(C and D) Dendritic groups from (A) summarized according to their position in an Eye-IPL map (cf. Figure 2). (C) summarizes major groups: Off (left, top) and On non-opponent (left, bottom); opponent (right, top); and On+Off non-opponent (right, bottom). (D) As (C), with opponent groups divided into their specific spectral computations as indicated. Note that most specific functions in (C) and (D) are restricted to specific regions of the eye and IPL. For example, green versus blue simple opponent computations occur mostly in the ON layers of the ventral retina that survey the world above the fish (D, bottom left).