Figure 5

Figure 5

Metabolic profiling following methiothepin treatment in ES2 and OV90 cells. (A) The metabolic profiling changes in ES2 and OV90 cells following methiothepin treatment were analyzed by seahorse analysis with oligomycin, FCCP, Antimycin A, and Rotenone treatment. (B) Basal OCR, proton leak, maximal respiration, and ATP production were analyzed as the metabolic parameters in ES2 and OV90 cells in response to treatment with methiothepin. (C) The energy phenotypes in ES2 and OV90 cells were obtained by seahorse assay following treatment with methiothepin. In ES2 and OV90 cells, oxygen consumption rate and extracellular acidification rate under baseline (open marker) and stressed (closed marker) conditions following methiothepin treatment were analyzed. Results are expressed as the means ± SDs of three independent experiments. The asterisks indicate statistically significant differences compared to the control (*** p < 0.001).