ZFIN Image: Ye et al., 2020, Fig. S3

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Figure Caption

Fig. S3

Validation of Tg(Δ113p53:CreER; β-act2:RSG) zebrafish. (A) Western blot with a monoclonal antibody against zebrafish Δ113p53 was performed to analyze the induction of Δ113p53 upon the treatment of a DNA damage drug, camptothecin (Campt). The Tg(Δ113p53:CreER; β-act2:RSG) zebrafish embryos at 1 day post fertilization (dpf) were treated with Campt for 24 hours. Afterwards, a part of untreated and Campt-treated embryos were divided and treated with 4HT for 2 hours. β-actin was used as the protein loading control. (B-E) Life images of red (DsRed) (B, C, D, E), green (EGFP) (B’, C’, D’, E’) and bright field (B”, C”, D”, E”) in Tg(Δ113p53:CreER; β-act2:RSG) zebrafish embryos treated with either Campt (C, C’, C”), or 4HT (D, D’, D”), or both (E, E’, E”) as described above. Scale bar, 500 μm.

Acknowledgments

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