Fig. 5

Confocal microscopy of dfG and s-GO injected in the spinal cord of zebrafish. (a) Images of dfG and s-GO dispersed in saline solution. Left panels for each material are bright field images, while right panels are images in reflective mode for dfG and in fluorescence for s-GO. (b) Z-stack reconstructions of the spinal cord of control and dfG-injected fish, 2 days after treatment. Images, acquired in fluorescence and reflective modes sequentially, show the localization of dfG (red) in the spinal cord, which is identifiable thanks to the presence of GFP positive motor neurons (green). (c and d) Z-stack reconstructions of the spinal cord of control and s-GO-injected fish, 2 days (c) and 1 day (d) after treatment. Images, acquired in fluorescence mode sequentially for the red and green channels, show the localization of s-GO (red) in the spinal cord, which is identifiable thanks to the presence of GFP positive motor neurons (green), only 1 day after the injection (d), but not later (c). In (d), below, magnification of the area in the square for s-GO injected fish.