Fig. 4

Effects of intra-spinal delivery of s-GO on zebrafish behaviour. (a) Left: Bright field image of recording chamber for free swimming test. Right: Swimming trajectories of 4 dpf control, dfG-injected and s-GO-injected zebrafish recorded over a 5 min period. (b) Plots reporting the values of the total distance swum, percentage of time spent swimming and swimming bout length. Dots superimposed to bars correspond to single experiments values. s-GO treatment significantly decreased the total distance swum and the length of swimming bouts (*P < 0.05). (c) Left, sketch of the experimental setting in startle response experiments. Right, swimming trajectories of 4 dpf control, dfG injected and s-GO-injected zebrafish swum after the application of an acoustic stimulus. (d) Plots reporting the values of the distance swum, the time spent swimming and the swimming velocity. s-GO treatment significantly decreased the distance swum and the time spent swimming (*P < 0.05).