Fig. 8.

Fig. 8.

Macrophages control Toxoplasma gondii burden in vivo. (A) Representative images of control (Ctrl; top) or macrophage-ablated (Mtz; bottom) mpeg:G/U:mCherry larvae infected in HBV with type I Toxoplasma-GFP (green). Individual larvae were imaged at 6 hpi and 24 hpi by fluorescent stereomicroscopy. Scale bar: 100 µm. (B) Automated enumeration of GFP-positive punctae in the HBV at 6 hpi and 24 hpi of Ctrl (gray open circles) or macrophage-ablated (open circles) larvae infected with type I Toxoplasma tachyzoites. Pooled data from three independent experiments with at least seven larvae per condition per experiment. Significance calculated using two-way ANOVA (repeated measures) with Sidak's multiple comparisons test. ***P≤0.001. p.i., post-infection. (C) Pixel volume quantification of individual GFP-positive punctae in Ctrl or macrophage-ablated larvae at 6 hpi and 24 hpi. Presented as percentage of total vacuoles counted in the HBV that are 1 tachyzoite/vacuole (<50 pix³), 2 tachyzoites/vacuole (50<100 pix³) or >4 tachyzoites/vacuole (>100 pix³). Pooled data from three independent experiments with at least three larvae per time point. Significance calculated using Chi-square test, χ²₂=1.248 (6 hpi), χ²₂=5.4 (24 hpi). ns, P>0.05. Mean±s.e.m. shown.